Widespread Nanoparticle-Assay Interference: Implications for Nanotoxicity Testing
نویسندگان
چکیده
منابع مشابه
Widespread Nanoparticle-Assay Interference: Implications for Nanotoxicity Testing
The evaluation of engineered nanomaterial safety has been hindered by conflicting reports demonstrating differential degrees of toxicity with the same nanoparticles. The unique properties of these materials increase the likelihood that they will interfere with analytical techniques, which may contribute to this phenomenon. We tested the potential for: 1) nanoparticle intrinsic fluorescence/abso...
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Laboratory Directors assume responsibility for the quality of data that is reported from their laboratories. A recent publication reminds us that the laboratory is likely to see contradictory data when comparing in-house interference data to information reported on the package insert (1). However, the quality of interference testing data provided by the manufacturer is seldom questioned, and, i...
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The advent of engineered nanoparticles (ENPs) and their increased use has remarkably improved the performance of basic materials and consumer products, but has also increased the potential for ENP release into the environment. Technical procedures, more accurate and specific than those for routine chemical analysis, are needed for characterization and quantification of ENPs at environmentally r...
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Aggregation is a common mechanism of compound-mediated assay interference encountered in high-throughput screening (HTS) and follow-up experiments. Compounds that form aggregates in situ can nonspecifically perturb biomolecules in biochemical and cell-based assays. Nonspecific bioactivity from aggregation can waste significant resources when unaccounted for in assay design and readout interpret...
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BACKGROUND Metal oxide nanoparticles (NPs) have been widely used in industry, cosmetics, and biomedicine. OBJECTIVES We examined hazards of several well-characterized high production volume NPs because of increasing concern about occupational exposure via inhalation. METHODS A panel of well-characterized NPs [cerium oxide (CeO₂NP), titanium dioxide (TiO₂NP), carbon black (CBNP), silicon dio...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0090650